固定化假单胞菌脂肪酶催化(R,S)-3-羟基丁酸乙酯转酯化拆分

编辑 2019-05-27
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附加信息

  • DOI(*): 10.16581/j.cnki.issn1671-3206.2015.09.012
  • EC: 3.1.1.3
  • 酶(*): 脂肪酶
  • pH(*):
  • 温度(*): 40°C
  • 介质(*): 甲苯
  • 催化剂类型(*): 固定化细胞
  • 酶物种来源或野生菌(*): 假单胞菌脂肪酶( Pseudomonas sp )
  • 产率(*): 15.6%
  • ee%(*): 96.2%

设计制备出比表面积290.6 m2/g、平均孔径和孔体积16.5 nm和1.66 cm3的功能化介孔载体G-NH2-MCF,G-NH2-MCF共价结合假单胞菌脂肪酶(Pseudomonas sp Lipase,PSL),获得了固定化酶PSL/G-NH2-MCF。在甲苯溶剂中,乙酸乙烯酯作酰化剂,固定化酶PSL/G-NH2-MCF催化(R,S)-3-羟基丁酸乙酯的转酯化拆分反应,转化率为15.6%,(S)-3-羟基丁酸乙酯的对映体过量值(eeS)为17.8%,(R)-3-乙酸基丁酸乙酯对映体过量值(eeP)为96.2%,对映选择性参数E为61;而在相同条件下,使用游离酶PSL催化拆分反应,转化率、eeS、eeP和E值分别为2.16%,1.95%,88.2%和16,PSL经G-NH2-MCF固定化后催化性能得到明显提高。同时研究了固定化酶PSL/GNH2-MCF催化(R,S)-3-羟基丁酸乙酯转酯拆分的反应条件对转化率和对映选择性的影响规律。

1.( R,S) -3-羟基丁酸乙酯的酶催化转酯化拆分

1.( R,S) -3-羟基丁酸乙酯的酶催化转酯化拆分.png

2.G-NH2-MCF 的制备和固定化酶示意图

2.G-NH2-MCF 的制备和固定化酶示意图.png

3.PSL/G-NH2-MCF 与游离酶PSL 催化作用比较

3.PSL G-NH2-MCF 与游离酶PSL 催化作用比较.png

4.反应时间对PSL /G-NH2-MCF 催化( R,S) -3-羟基丁酸乙酯拆分的影响

4.反应时间对PSL G-NH2-MCF 催化( R,S) -3-羟基丁酸乙酯拆分的影响.png

5.酰化剂对( R,S) -3-羟基丁酸乙酯拆分的影响

5.酰化剂对( R,S) -3-羟基丁酸乙酯拆分的影响.png

6.底物摩尔比对PSL/G-NH2-MCF 对映选择性的影响

6.底物摩尔比对PSL G-NH2-MCF 对映选择性的影响.png

7.溶剂对PSL/G-NH2-MCF 对映选择性的影响

7.溶剂对PSL G-NH2-MCF 对映选择性的影响.png

 

 

 

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