大肠杆菌全细胞转化联产L-2-氨基丁酸和D-葡萄糖酸

编辑 2019-05-28
给本项目评分
(0 得票数)

附加信息

  • DOI(*): 10.13345/j.cjb.170056
  • 酶(*): 脱氢酶
  • pH(*): 7.5
  • 温度(*): 30°C
  • 介质(*): 磷酸盐缓冲液
  • 催化剂类型(*): 全细胞
  • 酶物种来源或野生菌(*): 重组菌株BL21-LTD 和
  • 产率(*): 99%
  • ee%(*):

L-2-氨基丁酸 (L-ABA) 是一种非天然氨基酸,可以作为重要的前体物质合成多种手性药物,包括用于结核病治疗的乙胺丁醇和布瓦西坦,以及抗癫痫药物左乙拉西坦。多数报道均以酶法转化为基础,需要细胞破碎等繁琐步骤,并且需要外源添加一定数量的辅酶 (NAD+等)。本文构建了包括辅酶原位再生的偶联酶反应的途径,以L-苏氨酸为底物,D-葡萄糖为辅底物,以LTD 和LDH 一起构建包括辅酶NADH 循环再生的多酶催化体系,全细胞转化联产L-ABA 和D-葡萄糖酸。通过转化条件 (温度、pH、细胞通透性和菌体量) 优化,并采用分批补料策略,164 g/L L-苏氨酸和248 g/L D-葡萄糖最终转化得到141.6 g/L 的L-ABA 和269.4 g/L 的D-葡萄糖酸,时空得率分别达到7.1 g/(L·h) 和13.5 g/(L·h),得率超过99%。

1、温度 (A) 和pH (B) 对转化反应的影响:

SNRE{PW}WTJFTI@CB}R4]TO.png

2、不同细胞通透剂 (A) 和不同浓度曲拉通x-100 (B) 对转化反应的影响:

)L(P[3]$69H8TATKWBEK03N.png

3、BL21-LTD 和BL21-LDH/GDH 的转化效率 (A) 和菌体量浓度对转化反应的影响 (B):

BY{%QEE)0I082F9{7O@OY5M.png

4、5 L 发酵罐上进行全细胞转化联产L-ABA 与D-葡萄糖酸:

AH][RLZBRQQB1SX)AYQFS5M.png

5、生物合成L-ABA同类工作对比:

W__VUAJI_J8_DB}90B[077U.png

 

阅读 7201 次数

4396 评论

  • 评论链接 dronzlife 星期四, 21 11月 2019 23:17 发布人 dronzlife

    nike mercurial vapor x all white red billig miami heat fitted hats new era xolos billig adidas x 18.2 fg all white shoes nike bears 99 dan hampton whitepink womens stitched nfl limited rush fashion jersey billig nike cortez rouge noir pour vente 2014 super bowl jerseys denver broncos 88 demaryius thomas navy blue alternate limited jersey billig
    dronzlife http://www.dronzlife.com/

  • 评论链接 videobebin 星期四, 21 11月 2019 17:45 发布人 videobebin

    jordan 1 black og gum for cheap billig nike air max 2017 womens all black grey uk billig womens air jordan 5 bel air journal billig
    videobebin http://www.videobebin.com/

  • 评论链接 balenciaga 星期四, 21 11月 2019 14:05 发布人 balenciaga

    I抦 impressed, I need to say. Actually not often do I encounter a blog that抯 both educative and entertaining, and let me tell you, you could have hit the nail on the head. Your concept is excellent; the difficulty is something that not sufficient individuals are talking intelligently about. I am very joyful that I stumbled across this in my seek for something referring to this.

  • 评论链接 meehrzeit 星期四, 21 11月 2019 01:23 发布人 meehrzeit

    new era fitted dodger hats 2017 billig leicester city blank away mens adults 2016 2017 club soccer jerseys billig nike hypervenom 2 phinish black for cheap billig nike classic cortez black blue shoes nike kd trey 5 vi black blue shoes nike air force 1 3d stars billig
    meehrzeit http://www.meehrzeit.com/

  • 评论链接 ovguitars 星期三, 20 11月 2019 18:43 发布人 ovguitars

    nike kaishi herren all black billig 2014 15 manchester city 33 nastasic home soccer aaa t shirt womens billig nike free rn flyknit on foot billig women air max 2009 blue black shoes canada billig nike gs ii acc all black grey billig nike sf-af1 black little blue shoes
    ovguitars http://www.ovguitars.com/

  • 评论链接 lewislist 星期三, 20 11月 2019 10:22 发布人 lewislist

    new york yankees cap kopen kidney nike air max thea mens all red grey uk men nike st. louis rams 58 jo lonn dunbar elite navy blue team color nfl jersey sale nike magista onda fg nz instagram nike air foamposite one all black blue womens nike air max zero black grey shoes on sale
    lewislist http://www.lewislist.com/

  • 评论链接 yeezy shoes 星期二, 19 11月 2019 09:49 发布人 yeezy shoes

    Nice post. I learn something more difficult on different blogs everyday. It's going to all the time be stimulating to read content from other writers and follow a bit of one thing from their store. I抎 choose to use some with the content on my blog whether you don抰 mind. Natually I抣l offer you a hyperlink in your internet blog. Thanks for sharing.

  • 评论链接 kd shoes 星期一, 18 11月 2019 12:07 发布人 kd shoes

    Nice post. I study something more difficult on different blogs everyday. It would at all times be stimulating to learn content from different writers and observe a bit of one thing from their store. I抎 choose to make use of some with the content material on my blog whether or not you don抰 mind. Natually I抣l provide you with a link on your internet blog. Thanks for sharing.

  • 评论链接 лазерная эпиляция севастополь 星期六, 16 11月 2019 19:21 发布人 лазерная эпиляция севастополь

    https://club.foto.ru/nologin/650647 лазерная депиляция севастополь
    лазерная эпиляция бикини в саранске

    лазерная депиляция саранск
    http://www.nnov.org/profile/?user_id=7043961 лазерная депиляция в симферополе
    лазерная депиляция симферополь лазерная эпиляция в севастополе

    лазерная депиляция в севастополе
    http://puzyaka.ru/forum/member.php?u=59617
    лазерная депиляция севастополь

  • 评论链接 лазерная эпиляция в севастополе 星期六, 16 11月 2019 18:44 发布人 лазерная эпиляция в севастополе

    лазерная эпиляция бикини в севастополе
    лазерная депиляция симферополь лазерная эпиляция в саранске
    лазерная эпиляция в симферополе

    лазерная депиляция в севастополе
    лазерная эпиляция в саранске
    http://centerestetmedicina.com/about/forum/user/135396/
    лазерная депиляция саранск

写个评论

Make sure you enter all the required information, indicated by an asterisk (*). HTML code is not allowed.

酶催化广告

广告1

广告位small.jpg